Oxidative phosphorylation

Production of ATP via oxidation by the flavoprotein-cytochrome system.

• Hydrogen acceptors lose their hydrogen (i.e. oxidized)
• Transfers hydrogen to oxygen, forming water.
• Final enzyme is cytochrome c oxidase
  * Contains 2 atoms of Fe and 3 Cu

Occurs in mitochondria.

1. Protons pumped out of the matrix
   * i.e. out of the inner membrane of mitochondria
2. Proton concentration builts up in the intracristal space
   * i.e. between inner and outer membrane of the mitochondria
3. Transfer of protons back into the matrix drives an ATPase in the inner membrane.

ATP production

NADH = 3 ATPs

NADPH = 3 ATPs

FADH2 = 2 ATPs

Overall effect of glucose metabolism

For every molecule of glucose,

• 10 x NADH+H+
• 2 x FADH2
• 2 ATP directly in glycolysis
• 2 ATP/GTP in citric cycle

=> 38 ATPs + 6 CO2 + 6 H2O